Part:BBa_K4806200:Design

CYP3A4 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1846
  Illegal PstI site found at 2168
  Illegal PstI site found at 2228
  Illegal PstI site found at 2700
  Illegal PstI site found at 2769
  Illegal PstI site found at 2873
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 1846
  Illegal PstI site found at 2168
  Illegal PstI site found at 2228
  Illegal PstI site found at 2700
  Illegal PstI site found at 2769
  Illegal PstI site found at 2873
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1846
  Illegal PstI site found at 2168
  Illegal PstI site found at 2228
  Illegal PstI site found at 2700
  Illegal PstI site found at 2769
  Illegal PstI site found at 2873
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1846
  Illegal PstI site found at 2168
  Illegal PstI site found at 2228
  Illegal PstI site found at 2700
  Illegal PstI site found at 2769
  Illegal PstI site found at 2873
  Illegal NgoMIV site found at 2090
  Illegal NgoMIV site found at 3592
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2. CYP3A4 gene (B3-B4) (BBa_K4806000)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.